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Journal of Stem Cell Research and Tissue Engineering
Published by Universitas Airlangga
ISSN : 26141264     EISSN : 26141256     DOI : https://dx.doi.org/10.20473/jscrte
Core Subject : Health, Science, Engineering,
Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary
Journal of Stem Cell Research and Tissue Engineering (JSCRTE) is published by Stem Cell Research and Development Center, Airlangga University. Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancerstem cells, developmental studies, genomics and translational research. Special focus of JSCRTE is on mechanisms of pluripotency and description of newly generated pluripotent stem cell lines. Articles that go through the selection process will be review by peer reviewer or editor. The journal is published regularly twice a year in December and May. Every publication consists of 60-70 pages and 5 scientific articles in the form of research, study literature, and the case study in English. The contributors Journal of Stem Cell Research and Tissue Engineering are Stem Cell researchers, lecturers, student and practitioners that came from Indonesia and abroad.
Arjuna Subject : Kedokteran - Imonologi dan Alergi
Articles 5 Documents
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Search results for , issue "Vol. 4 No. 1 (2020): Journal Of Stem Cell Research and Tissue Engineering" : 5 Documents clear
Calculation Of Coliform Number Using Most Probable Number (MPN) Methods On Soy Milk Sold in Pogot Area Of Surabaya racma nisaa
Journal of Stem Cell Research and Tissue Engineering Vol. 4 No. 1 (2020): Journal Of Stem Cell Research and Tissue Engineering
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v4i1.21591

Abstract

The quality of drinks is one of the problems that exist in society, especially because of bacterial contamination of drinks that often occurs in developing countries. One of them is soy milk drinks that are contaminated by bacteria including those sold by street vendors in the Pogot area of Surabaya. Based on the background description in this study can be formulated as follows: Is the number of Most Probable Number (MPN) Coliform in soy milk sold by street vendors in the Pogot area of Surabaya fulfill the Indonesian National Standard (SNI) 7388: 2009? The purpose of this study was to determine the amount of Coliform in soy milk sold by street vendors in the Pogot area of Surabaya compared to the Indonesian National Standard (SNI) 7388: 2009 regarding the maximum limit of microbial contamination in food. This study used a descriptive study design that soy milk sold by street vendors in the Pogot area of Surabaya was the population. Samples were used as many as 16 samples from 4 street vendors, each of which was taken 4 then laboratory-tested and analyzed using percentages. Based on the requirements of the Indonesian National Standard (SNI) 7388: 2009, the limit of contamination of the Most Probable Number (MPN) Coliform in soy milk is 20 / ml. Based on the results of the research of 4 soy milk traders there were 2 traders whose the results fulfilled the requirements of the Indonesian National Standard (SNI) and 2 traders whose the results didn't  fulfill the requirements of the Indonesian National Standard (SNI). So 50% were eligible (MS) and 50% were not eligible (TMS).
Isolation and Identification Avian Influenza A non-H5 Virus from Muscovy Duck (Cairina moschata) at Two Live Bird Markets in Surabaya deya karsari
Journal of Stem Cell Research and Tissue Engineering Vol. 4 No. 1 (2020): Journal Of Stem Cell Research and Tissue Engineering
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v4i1.21597

Abstract

The aim of this study was to isolate and identify Avian Influenza A non-H5 virus from muscovy duck at two live bird markets in Surabaya. Muscovy duck is the natural reservoir of Avian Influenza virus, in which all of the 16 HA subtypes and 9 NA maintained. The Avian Influenza virus replicates in intestinal tract of the reservoirs, causing the high amount of virus shed in the faeces. This study is an observational descriptive study, using non random sampling method of determined samples. The  method used in this study were Hemagglutination Inhibition (HI) test and Enzyme Linked Immunosorbent Assay (ELISA) test. Avian Influenza A non-H5 virus was identified 19.23% (5 samples out of 26) in PS1 and  23.34% (7 samples out of 30)  in PS2. This finding shows that  Avian Influenza A non-H5 virus could be isolated and identified from muscovy duck at two live bird markets in Surabaya.
Serotype Infectivity and Phylogenetic of Dengue Virus cause of Dengue Fever (DF), Dengue Hemorrhagic Fever (DHF), and Dengue Shock Syndrome (DSS) in Surabaya- Indonesia fedik rantam
Journal of Stem Cell Research and Tissue Engineering Vol. 4 No. 1 (2020): Journal Of Stem Cell Research and Tissue Engineering
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v4i1.21588

Abstract

Infection with DENV causes a spectrum of clinical disease ranging. The aim of this study is to investigate the infectivity of DENV with degree of severity dengue infection in Surabaya. Dengue infection was established by IgM anti dengue, and two step multiplex RT PCR and Nucleotide sequence. Grading of degree severity infection follow the WHO criteria 2011. DSS cases found 3 from 36 patients caused by DENV 2. The most uninfective was DENV 1, and the most prevalence dengue infection caused  by  DENV  3.  The  infectivity of  dengue  infection shown 16 patients lead to severity with plasma leakage. All of sera patients detecting using multiplex RT-PCR were positive, but it were analyzed using Duo ELISA only 22 serum sera positive IgM and IgG from 36 sera. . The Phylogenetic analysis indicates that the isolates from 2011 to 2012 close related with dengue isolate from 1998 and belong to 2009 to 2020.In this study it indicates that DENV 2 predominantly is the cause of DSS.
The effect of Extreme Low Frequency-Pulsed Electromagnetic Field exposure in the healing process of Sprague Dawley mouse delayed union femur fracture: study of Rust Radiology Score andika djaja
Journal of Stem Cell Research and Tissue Engineering Vol. 4 No. 1 (2020): Journal Of Stem Cell Research and Tissue Engineering
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v4i1.21589

Abstract

Fractures usually heal normally. In some conditions, the healing process do not occur normally, but become delayed union or non union as a complication. Successful healing of fractures is a complex interaction between the process of angiogenesis and osteogenesis (the interaction of osteoblasts and osteoclast). Physical stimuli such as exposure of EMF (electromagnetic fields) influences of the osteogenesis process both in the development stage of embryo reinforcement and the fracture healing stage. The aim of this study is to determine the healing of delayed union fractures in experimental animals due to the expossure of Extreme Low Frequency-Pulsed Electromagnetic Field (ELF-EMF) by comparing the RUST scores. The experimental study was conducted Department of Nutrition Animal Laboratory, Faculty of Medicine, Universitas Indonesia with 56 experimental rats during August-September 2018. There were no differences of animal characteristics in the study. It was found that there were significant differences in Rust Score in the treatment and control groups in each examination week. There were no differences in clinical improvement in the two groups.  This study concluded that there was an improvement in delayed union fracture healing after the administration of ELF-EMF as seen from the difference in Rust score.
The Osteogenic Capacity of HumanAmniotic Membrane Mesenchymal Stem Cell (hAMSC) and Potential for Application in Maxillofacial Bone Reconstruction in Vitro Study David Kamadjaja
Journal of Stem Cell Research and Tissue Engineering Vol. 4 No. 1 (2020): Journal Of Stem Cell Research and Tissue Engineering
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v4i1.21590

Abstract

Amniotic membrane of human placenta is a source of abundant mesenchymal stem cell (hAMSC) which makes it a potential source of allogeneic multipotent cell for bone healing.  However, much has to be explored about its isolation procedure and the osteogenic differentiation potential. The aims of this study are to establish the procurement procedure of human amniotic membrane, the isolation   and culture   of hAMSC, the MSC phenotypic characterization, and the in vitro osteogenic differentiation of hAMSC.  Results of the study are as follows. The quality of human amniotic membrane would be best if procured from Caesarean operation under highly aseptic condition to avoid fungal and bacterial contamination on the culture.  Isolation procedure using modified   Soncini protocol yielded large amount of MSC with high proliferative capacity in culture medium.  Characterization of hAMSC showed that the majority of the target cells exhibited specific MSC markers (CD10S and CD90) with a small number of these cells expressing CD45the marker of hematopoeitic cells. The in vitro osteogenic differentiation of hAMSC  followed by Alizarin  Red staining showed that  osteoblastic differentiation  was  detected in a significantly   high  number  of cells.  This study concludes that hAMSCs isolated from human amniotic membrane have the capacity for in vitro osteogenesis which makes them be one of the potential allogeneic stem cells for application in maxillofacial bone reconstruction.

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